报告时间：2019年11月15日下午3:00

报告地点：近代物理系409会议室

报告题目：Science and technology of artificial cell reactor 

报告人：Prof. Hiroyuki Noji, The University of Tokyo

报告摘要： We developed femto-liter reactor array device (FRAD) that display over million water-in-oil droplets with size of femto-liter range. One can readily encapsulate bio- or non-biological molecules into the FRAD reactors, by spreading assay mixture on the device and sealing the reactors with oil. The extremely small volume features of the reactors on FRAD allows very sensitive bioassay at single-molelule referred as to ‘digital bioassay’. The massive number of the reactor enables to select highly active but very rare functional molecules among a large numbers of library. Several examples will be introduced including Digital ELISA, Digital Influenza Virus Counting, and single-transporter analysis. The high biocompatibility of FRAD reactors also allows the reconstitution of molecular systems such as cell-free gene expression and replication. In the last part of the presentation, I will introduce the concept of FRAD in the context of synthetic biology, and show digital gene expression with an application for the engineering of alkaline phosphatase to enhance catalytic rate constant. 

报告人简介：

Professor Hiroyuki Noji is a single-molecule biophysicist. He has been studying the chemomechanical coupling mechanism of ATP synthase by the use of single-molecule techniques. His work to visualize the rotary motion of F1-ATPase of ATP synthase is featured in many prominent textbooks such as The Cell, Biochemistry by Stryer. He is also known as an inventor of the femtoliter chamber array system for Single-Molecule Enzymatic assays that is currently applied in single-molecule digital ELISA. After development of femto-liter reactor array device (FRAD), he is actively expanding the application of FRAD towards artificial cell studies including enzyme screening and cell reconstitution. He is also the program manager of Impulsing Paradigm Change through Disruptive Technologies Program (ImPACT), ‘Artificial Technology, of Japan Agency of Science and Technology (JST). He has been awarded Yomiuri Gold Medal Prize from Yomiuri foundation 2015, Nakatani Incentive Prize from Nakatani foundation 2015, Inoue Science Research Award from Inoue Foundation for Science 2013, JSPS Prize from Japan Society for the Promotion of Science 2006, Amersham Pharmacia Biotech & Science Prize for Young Scientists from American Association for the Advancement of Science (AAAS) and Amersham Pharmacia Biotech, 1998. 

Research Interests: Single-molecule biophysics of ATP synthase; Digital bioassays; Cell-free synthetic biology